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局部荧光探针可以检测肠癌啦！

来源：Chemistry ＆biology 发布者：管理员时间：2015/1/27 14:11:00

近日，国际化学与生物学杂志chemistry＆biology发表了来自斯坦福大学医学院Ravindra Majeti研究小组的一项最新研究成果，他们发现一种荧光淬灭探针（aABP）能够特异性靶向在肠道发育不良中高表达的半胱氨酸蛋白酶，对指示肠道肿瘤具有非常高的敏感性和特异性。

早期检测结肠息肉能够预防高达90%的结肠癌病人死亡，传统的结肠镜检查能够便捷检测大多数的已表现出特定形态的癌前病变，但对于潜伏病变，结肠镜检查并不能有效检测。因此基于分子差别开发检测试剂提高肠癌的检测率变得十分必要。

之前研究发现半胱氨酸蛋白酶在自发性肠息肉和化学诱导结肠癌导致的肠道不良发育中具有非常高的表达水平。本文研究人员针对检测半胱氨酸蛋白酶发现了一种荧光淬灭探针（aABP），能够选择性靶向多种半胱氨酸蛋白酶，并且发现aABP通过靶向半胱氨酸蛋白酶，提高了对人和小鼠肠道肿瘤识别的敏感性和特异性。除此之外，aABP能够通过静脉注射给药或结肠部局部给药,减少病人检测时的痛苦。

综上所述，该文章发现了一种特异性识别半胱氨酸蛋白酶的荧光探针，并提出应用荧光引导下结肠镜检查技术能够有效提高对肠道肿瘤的检测，预防结肠癌。这一发现或许对临床检测肠道肿瘤具有非常重要的意义。（转自生物谷）

原文如下：

dx.doi.org/10.1016/j.chembiol.2014.11.008

Detection of Intestinal Cancer by Local, Topical Application of a Quenched Fluorescence Probe for Cysteine Cathepsins

Ehud Segal, Tyler R. Prestwood, Wouter A. van der Linden, Yaron Carmi, Nupur Bhattacharya, Nimali Withana, Martijn Verdoes,Aida Habtezion, Edgar G. Engleman, Matthew Bogyo

Early detection of colonic polyps can prevent up to 90% of colorectal cancer deaths. Conventional colonoscopy readily detects the majority of premalignant lesions, which exhibit raised morphology. However, lesions that are flat and depressed are often undetected using this method. Therefore, there is a need for molecular-based contrast agents to improve detection rates over conventional colonoscopy. We evaluated a quenched fluorescent activity-based probe (qABP; BMV109) that targets multiple cysteine cathepsins that are overexpressed in intestinal dysplasia in a genetic model of spontaneous intestinal polyp formation and in a chemically induced model of colorectal carcinoma. We found that the qABP selectively targets cysteine cathepsins, resulting in high sensitivity and specificity for intestinal tumors in mice and humans. Additionally, the qABP can be administered by either intravenous injection or by local delivery to the colon, making it a highly valuable tool for improved detection of colorectal lesions using fluorescence-guided colonoscopy.